FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37437409
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA129150.011.8425524
GACATAA171950.011.7386725
CAACGAT179700.011.3856981
ACGATCG64000.011.2840723
GCAAAAG343900.011.2070711
ACGATTC138650.010.8285283
AGACATA217700.010.3638014
CGATCGA55550.010.0897834
AAGGGGG410450.09.88756380-81
CGAGAAT95900.09.873561
TAACCGA146400.09.41043116-17
AACGATT188250.09.3686842
AAAAGGG408750.09.09203178-79
CGGGCAT137800.08.9086071
TAATGGA219100.08.6708229
GATTCAA228650.08.661585
CGAAACG159300.08.61895126-27
CCGGGAT110700.08.5964991
TCGGTTA35100.08.59245292-93
CAAAAGC423650.08.5504192
Produced by FastQC (version 0.11.5)