FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37072128
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT13800.010.6880651
AAAGGGG447000.010.56662680-81
AGGGGGG630500.09.61185682-83
TAGGGGG162100.09.33319350-51
TAAAAAA1076750.09.28243670-71
CGGGAAT76650.09.1868381
AAAAAGG620450.08.88329178-79
AAAAGGG466650.08.78324678-79
AAGGGGG452950.08.76587180-81
GTTCGAT43800.08.0384831
CGGGCAT146500.08.0218731
CGTACGT60350.07.949786732-33
GGGGGGA597950.07.9181412
GTCCGAT33300.07.8584151
CGCGGAT55400.07.72946171
GCGTCGT291700.07.701627394-95
CCGGGAT116400.07.52109961
ATCGAGT77050.07.5199126
CGACGAT72900.07.5056241
CGCGAGT38150.07.4829561
Produced by FastQC (version 0.11.5)