FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33885201
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG455950.012.10904480-81
AAAAGGG475200.010.11928578-79
AAGGGGG472400.010.07376880-81
AAAAAGG629500.09.97771678-79
TAAAAAA1068800.09.8313670-71
AGGGGGG735250.09.66017882-83
TAGGGGG170950.09.48897250-51
CGGGGAT102300.08.9771571
GGGTTAA104600.08.58146466-67
CGGGAAT71750.08.2235041
AAAAAAG967900.08.17467776-77
CGGGCAT135900.08.1581861
CCGGGAT108750.08.0946781
GTCGATT42650.08.0328671
CGAATCG29450.07.74405153
GGCGAAT43100.07.50738671
CGGGGTT123750.07.45956751
GGATCGA68150.07.4573974
ATCGGAA578650.07.45673294-95
GATCGGA622700.07.34110594-95
Produced by FastQC (version 0.11.5)