FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36228836
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG485800.011.46786780-81
AGGGGGG741550.09.62632882-83
TAAAAAA1016300.09.528625570-71
CGGGAAT70500.09.4489631
AAGGGGG473650.09.19504680-81
AAAAAGG692400.09.0406878-79
GTCGATT38900.08.8070021
AAAAGGG515150.08.7216878-79
CGCGATT12151.2732926E-108.6157211
CGCGTAT6655.602257E-58.5862751
CGGGGAT140800.08.3809691
CGCGAAT13157.4578566E-118.3223751
GGCGAAT75150.08.2944491
TAGGGGG155500.08.21699850-51
AAAAAAG913050.08.14586676-77
CCGGGAT106700.08.0271
GGGTTAA113150.07.78630166-67
TCGAACG34500.07.71609452
CTCGGGT94250.07.6737461
GCGAGTT84400.07.6672871
Produced by FastQC (version 0.11.5)