FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37771811
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT7251.409353E-810.5008621
AGGGGGG630700.09.95886582-83
AAAGGGG472500.09.90083380-81
AAGGGGG439600.09.11307180-81
TAAAAAA1062800.09.01130670-71
TCGAACG38150.08.97176552
CGGGAAT76500.08.832221
TAGGGGG152650.08.44824350-51
GGCGAAT77300.08.3099281
CGCGAAT14901.8189894E-128.3029051
GTCGATT39000.08.2963531
CGCGATT13401.10958354E-108.1670661
CGGGGTT117750.08.1626921
AAAAAGG689950.08.09864878-79
AAAAGGG508650.08.0907378-79
CGGATAC73600.08.0047743
ACGGATA58400.07.97724962
CGGGCAT138500.07.8673541
GTCGGAT37550.07.85642051
CGAACGA43450.07.76379063
Produced by FastQC (version 0.11.5)