FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27664992
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG498900.1803362169777602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT122850.08.3279221
GGGAAAT206150.07.8712611
CGGGAAT69500.07.46302651
CGCGATT19400.07.35856061
CGGAAAT66150.07.33742431
CTCGGAT82050.07.307441
CTCGTAT32000.07.28650761
GGAAAAT239600.07.09013841
CGAATCG20800.07.0816093
CGGGATT65650.07.030891
GGCGAAT46550.06.95124441
ATCGAAC26800.06.91139276
CCGGGAT105600.06.89446971
CTGGAAT241350.06.86128231
CGCGAAT17354.965841E-106.85667941
GGGGAAT154100.06.82437561
CCGGAAT63550.06.81395341
AATCCAA154850.06.7782385
TCGGATC47500.06.70594362
AAATCGT51750.06.70057154
Produced by FastQC (version 0.11.5)