FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n02_HBHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n02_HBHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34103315
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG456250.012.28853580-81
TAGGGGG160300.012.03041950-51
AGGGGGG684200.010.44005482-83
TAAAAAA1020600.010.14227670-71
AAAAAGG627300.010.09994878-79
AAGGGGG476400.09.83971280-81
AAAAGGG477900.09.83867878-79
CGGGGAT108300.09.4025391
CGCGTAT7301.8042494E-58.4738421
GTTCGAT37500.08.3747631
AAAAAAG947300.08.36518876-77
GGGTTAA109350.08.16535666-67
GTCGATT41600.08.0069021
CGGAAAT81950.07.89677521
CGGGAAT74350.07.871981
CCGGGAT114900.07.78571
CGGGATT69850.07.7660161
GGGGGGA642400.07.725712
CCGGTAT48250.07.59369661
CCGGAAT71650.07.5709171
Produced by FastQC (version 0.11.5)