FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35164198
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8198992.3316300289288554No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3095310.8802447307343679TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1017500.061.4075938
CGGAAGA1088800.057.7227674
TCGGAAG1089450.057.5278663
ATCGGAA1102950.056.845892
GATCGGA1091250.055.2560041
GAGCACA1156600.054.026449
AAGAGCA1266450.049.8761947
GAAGAGC1310500.047.929466
GGAAGAG1415250.044.6403585
TATCTCG416300.042.960840-41
CTCGTAT499500.042.3987644-45
TCTCGTA503450.041.94225742-43
ATGCCGT553250.040.73858348-49
CGCTCAT490150.039.35425634-35
ATCTCGT532250.039.07943342-43
CACCGCT492150.038.60501530-31
CGTCTGA809000.038.19579716-17
CCGCTCA505150.038.00044332-33
TCACCGC502400.037.98284530-31
GTCACCG506750.037.8503228-29
Produced by FastQC (version 0.11.5)