FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41089568
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4717971.1482160143421318TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1256750.045.0978559
CGTATGC614150.044.51766646-47
TCGTATG606150.044.50422744-45
TATGCCG617650.044.2655948-49
CTCGTAT582650.044.1633944-45
AGAGCAC1330200.042.618398
TCTCGTA587950.041.9063742-43
GTCACGA681050.040.95845828-29
ATCGGAA1390250.040.454862
CGGAAGA1400900.040.336354
GTATGCC676700.040.2693646-47
TCGGAAG1403750.040.1938933
ATGCCGT688950.039.4190748-49
GCCGTCT684900.039.23301350-51
ATCTCGT631200.038.82047342-43
AGTCACG723350.038.78327628-29
CGAGATT719300.038.72872532-33
TCACGAG725100.038.71093430-31
GATCGGA1425100.038.589371
CACGAGA723700.038.58563630-31
Produced by FastQC (version 0.11.5)