FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35790827
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3246590.9071011407476TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG419650.044.2042644-45
CGTATGC427200.044.02462846-47
CTCGTAT405950.043.50808344-45
GAGCACA912250.042.8602839
TATGCCG445900.042.24769248-49
GTCACGA474000.040.60568228-29
TCTCGTA415250.040.54257242-43
AGAGCAC971700.040.2673538
ATCGGAA985250.039.377212
TCGGAAG1004050.038.7483833
GTATGCC487300.038.54134446-47
CGGAAGA1008950.038.527144
AGTCACG507550.038.0993828-29
ATGCCGT492650.038.05541248-49
TCACGAG510200.037.97004730-31
GATCGGA1013350.037.7579081
GCCGTCT492000.037.71012550-51
CACGAGA515600.037.3881330-31
ATCTCGT451200.037.0491142-43
CGAGATT523600.036.80291432-33
Produced by FastQC (version 0.11.5)