FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35630912
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4380361.229370721692445TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1065200.048.5169149
TCGGAAG1074800.047.9785233
AGAGCAC1091250.047.2934468
CGGAAGA1092300.047.2271084
ATCGGAA1110000.046.3291932
GATCGGA1103150.045.984111
TCGTATG551500.044.62095344-45
CGTATGC562100.044.4694546-47
CTCGTAT531750.044.17468644-45
TATGCCG572100.043.62185348-49
AAGAGCA1212450.042.6716277
TCTCGTA540900.041.5035242-43
GTCACGA615600.041.41650828-29
GTATGCC621850.040.1278946-47
TCACGAG639450.040.0894430-31
GAAGAGC1301150.039.8655056
ATGCCGT627700.039.493148-49
CGAGATT646750.039.33497232-33
AGTCACG654100.039.2256628-29
CCGTCTT629500.039.18079452-53
Produced by FastQC (version 0.11.5)