FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34617801
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4263091.2314733682824046TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1029950.051.400943
GAGCACA1037850.050.9719629
CGGAAGA1047200.050.5677724
AGAGCAC1062700.049.739828
ATCGGAA1063600.049.5878072
GATCGGA1048650.049.4067761
AAGAGCA1175650.045.041897
TCGTATG565550.044.77703544-45
CGTATGC576700.044.43601246-47
TATGCCG580000.044.08519448-49
CTCGTAT543600.044.07299844-45
GTCACGA623750.042.05208628-29
GAAGAGC1263900.042.0066156
TCTCGTA544350.041.89511542-43
TCACGAG651650.040.48630530-31
GTATGCC632950.040.41195346-47
ATGCCGT631800.040.2489548-49
CGAGATT655350.039.9017232-33
AGTCACG662050.039.7807828-29
CCGTCTT634850.039.59117552-53
Produced by FastQC (version 0.11.5)