FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32485894
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3143160.9675460986236056TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA800000.047.9311832
GATCGGA810000.046.84881
TCGGAAG827700.046.4700783
GAGCACA847300.045.629019
CGGAAGA848400.045.4030884
AGAGCAC853850.045.2011078
TCGTATG413500.043.97133344-45
CGTATGC419550.043.93866346-47
TATGCCG423650.043.36794748-49
CTCGTAT395550.043.34944-45
TCTCGTA407100.040.29796242-43
GTCACGA470650.040.11256428-29
AAGAGCA988500.039.1689577
GTATGCC477300.038.4581946-47
TCACGAG495900.038.32230830-31
ATGCCGT478150.038.14667548-49
CGAGATT499100.037.7476132-33
AGTCACG512000.037.20234728-29
CACGAGA511900.036.87397830-31
CCGTCTT494250.036.65930652-53
Produced by FastQC (version 0.11.5)