FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35675590
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3909631.09588376814511TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA934900.050.5256842
TCGGAAG958100.049.4552843
GATCGGA941600.049.2874761
GAGCACA968850.048.9740839
AGAGCAC973150.048.6356668
CGGAAGA988250.047.984734
TCGTATG505700.044.5580844-45
CGTATGC517100.044.3210746-47
CTCGTAT491200.043.96362744-45
TATGCCG522950.043.79839348-49
AAGAGCA1132350.041.96157
GTCACGA568550.041.1305228-29
TCTCGTA503500.041.0018742-43
TCACGAG595700.039.597230-31
ATGCCGT580850.039.2362448-49
GTATGCC584250.039.13359546-47
GAAGAGC1217500.039.1132626
CGAGATT597650.039.08969532-33
AGTCACG613350.038.40508728-29
CACGAGA615300.038.0386530-31
Produced by FastQC (version 0.11.5)