FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34295375
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3841041.1199877534507205TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG469550.044.46319644-45
CGTATGC476700.044.1614946-47
TATGCCG477100.043.9106748-49
CTCGTAT460850.043.8854244-45
GAGCACA1002750.043.2213489
TATCTCG472750.042.46795340-41
AGAGCAC1039700.041.6761638
TCTCGTA485750.041.22416742-43
CGGAAGA1065300.040.57714
TCGGAAG1068100.040.4440423
ATCGGAA1073150.040.2013742
ATGCCGT524950.039.6276448-49
GTATGCC528400.039.55745346-47
GATCGGA1106850.038.6460841
CCGTCTT542150.038.30921652-53
GCCGTCT539950.038.26319550-51
ATCTCGT528200.038.22132542-43
ACCGCTC558600.038.1319732-33
AAGAGCA1160500.037.3666657
TGCCGTC560600.037.22233250-51
Produced by FastQC (version 0.11.5)