FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37437409
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4084381.0909889624038887TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG489750.044.3891144-45
CGTATGC497000.044.22594546-47
CTCGTAT482300.043.81422444-45
TATGCCG501500.043.71091548-49
TATCTCG495850.042.2952640-41
GAGCACA1094350.041.339719
TCTCGTA511450.040.95881342-43
AGAGCAC1130300.039.9198158
ATGCCGT554300.039.3158548-49
GTATGCC560850.039.03859346-47
CGGAAGA1167450.038.5446554
TCGGAAG1169300.038.4757043
ACCGCTC580500.038.2233532-33
ATCTCGT553300.038.1483842-43
ATCGGAA1176600.038.1323362
GCCGTCT569450.037.9949350-51
CCGTCTT573500.037.87211652-53
TGCCGTC584400.037.44967350-51
CGCTCAT597600.037.32428734-35
CACCGCT603900.036.6443930-31
Produced by FastQC (version 0.11.5)