FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37072128
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3847171.0377526750015538TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG473950.043.78452744-45
CGTATGC480350.043.5289646-47
TATGCCG480850.043.28645748-49
CTCGTAT465250.043.276144-45
GAGCACA1059150.041.0049069
TCTCGTA489450.040.7958642-43
TATCTCG492400.040.7202340-41
AGAGCAC1086100.039.8912248
ATCGGAA1113800.038.5126382
ATGCCGT539150.038.469248-49
GTATGCC545050.038.21372246-47
TCGGAAG1133400.037.9510463
CGGAAGA1136950.037.8992354
ACCGCTC563100.037.54016532-33
CCGTCTT555800.037.1034652-53
ATCTCGT544600.036.9262342-43
GCCGTCT565100.036.4088850-51
CGCTCAT585000.036.38257634-35
GATCGGA1161100.036.3241731
TGCCGTC579400.035.94478250-51
Produced by FastQC (version 0.11.5)