FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33885201
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4297821.2683472056134477TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1037100.046.3971069
AGAGCAC1058350.045.6360638
TCGTATG520450.044.622744-45
CGTATGC527550.044.2895846-47
CTCGTAT512400.044.01672744-45
ATCGGAA1090700.043.9833952
TATGCCG529550.043.93433448-49
TCGGAAG1110150.043.374873
CGGAAGA1116900.043.0956574
GATCGGA1121650.042.355221
TATCTCG531500.042.24640340-41
TCTCGTA536550.041.72933642-43
AAGAGCA1215850.039.763537
GTATGCC587250.039.6900346-47
ATGCCGT583400.039.68769548-49
ACCGCTC604600.039.12766332-33
CCGTCTT597750.038.55298252-53
GCCGTCT602300.038.166950-51
CGCTCAT627550.037.8781334-35
CACCGCT628050.037.63329330-31
Produced by FastQC (version 0.11.5)