FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36228836
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4170161.1510609946176575TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1030500.045.357129
TCGTATG507000.044.36363244-45
CGTATGC514400.043.9530546-47
TATGCCG513800.043.85220348-49
ATCGGAA1067350.043.4149742
CTCGTAT502750.043.29791644-45
AGAGCAC1078750.043.2447478
TATCTCG510850.042.4335940-41
GATCGGA1092700.042.0370251
TCGGAAG1108550.041.924643
CGGAAGA1114300.041.746494
TCTCGTA528350.040.9564742-43
GTATGCC580300.038.85524446-47
ACCGCTC594950.038.34844232-33
GCCGTCT589400.037.78455750-51
ATGCCGT597400.037.5445948-49
ATCTCGT579700.037.5374642-43
CGCTCAT614650.037.3276834-35
TGCCGTC605200.037.17876450-51
CCGTCTT602050.037.04625352-53
Produced by FastQC (version 0.11.5)