FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37771811
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3889241.029667335781173TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG493400.044.0474344-45
CGTATGC499900.043.9226746-47
TATGCCG499400.043.8527448-49
CTCGTAT486500.043.26134544-45
GAGCACA1048900.043.2584849
TATCTCG497400.041.9252140-41
TCTCGTA511300.040.69285242-43
AGAGCAC1119800.040.4814038
ATCGGAA1114300.040.426962
GATCGGA1141000.039.099961
TCGGAAG1155800.039.065193
CGGAAGA1161550.038.977974
GTATGCC565950.038.66232346-47
ACCGCTC587100.037.95200732-33
GCCGTCT574250.037.43450-51
ATGCCGT584450.037.13848-49
ATCTCGT566800.037.0099642-43
CGCTCAT603950.036.9987734-35
TGCCGTC595150.036.70604750-51
CCGTCTT592550.036.56572352-53
Produced by FastQC (version 0.11.5)