FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27664992
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1549940.5602531893014825TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG195950.042.27469644-45
CGTATGC199500.041.5717546-47
CTCGTAT201200.040.12106344-45
TATGCCG206750.039.9534748-49
TATCTCG206350.039.07236540-41
TCTCGTA223100.036.21354742-43
ATCGGAA480250.035.239432
GATCGGA480450.035.1025851
TCGGAAG498350.034.072933
GTATGCC252950.032.96575546-47
GAGCACA522600.032.8731049
AGAGCAC522250.032.7868
ATGCCGT254100.032.56446548-49
CGGAAGA524600.032.3950274
ACCGCTC261850.031.91640932-33
ATCTCGT261550.030.99881642-43
CCGTCTT266800.030.8176852-53
CGCTCAT273700.030.60373534-35
GCCGTCT270100.030.5739950-51
CACCGCT274450.030.56392330-31
Produced by FastQC (version 0.11.5)