FastQCFastQC Report
Sat 8 Jun 2019
HMJHVBGXB_n01_HBHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJHVBGXB_n01_HBHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34103315
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4398191.2896664151270925TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1041100.046.762389
AGAGCAC1047050.046.3696258
TCGGAAG1061550.045.7199673
ATCGGAA1061150.045.634782
CGGAAGA1085100.044.740444
GATCGGA1072100.044.6083221
TCGTATG523900.044.45657344-45
CGTATGC532350.044.12694546-47
TATGCCG535450.043.6500848-49
CTCGTAT519300.043.38691744-45
TATCTCG531550.042.2967440-41
AAGAGCA1185200.041.0650947
TCTCGTA544900.041.05572542-43
GTATGCC594950.039.38815346-47
ATGCCGT594900.039.2161548-49
ACCGCTC614950.038.59228532-33
CCGTCTT605100.038.2682652-53
GCCGTCT608800.038.0634450-51
CGCTCAT630750.037.780134-35
ATCTCGT596950.037.70668442-43
Produced by FastQC (version 0.11.5)