FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41633971
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20786384.992648911630361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14152123.3991761199045847No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN605220.14536686880048028No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534940.12848642278201136No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN474940.11407511428587969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT381950.036.4116941
ATACGAG111400.030.9892735
GGGTTCT190600.030.8423753
TGTACTC71200.028.6639562
TTGTACT74200.028.6313271
CGGGTTT213250.026.908281
GGGGTTC253600.025.9535392
CCGGGGT293200.022.9074251
GTACTCT97950.021.1208363
TCTAGTT101950.020.9352237
CCGGGTT262400.018.8633271
GTTCTTT286450.017.899555
ACGAGAT235250.017.1854367
CGGGGGT677150.017.0991
CCGGGAA208350.016.9591581
CGGGAAC204400.016.6530592
CCATTGT145050.015.014069145
GGAACGT220750.014.9758254
GAACGTA244250.014.9688145
GGGAACG263350.013.7750513
Produced by FastQC (version 0.11.5)