FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_9424.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_9424.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21214209
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA1841300.8679560006220358No Hit
CGAGTAATGGGGGATAACTAATCGAAAGATTAGCTAATACCGCATATTCT509140.2399995210757092No Hit
CGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGG507590.23926887870294858No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG459760.21672266922608333No Hit
GTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCACAGA395520.18644107824147485No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC371240.1749959190088115No Hit
CTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGT355150.16741137979738016No Hit
CGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGT303460.14304563512125293No Hit
GCGGTGTCCTACTCTCCCACAACCCATCGGTTGCAGTACCATCGGCGCTG286800.13519240806951605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270050.12729675662194145No Hit
GGGGGATAACTAATCGAAAGATTAGCTAATACCGCATATTCTCTGAGGAG234300.11044484383084942No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC218240.10287444608469729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG596800.050.171183
CTTACAT152400.049.7386441
TGGGTTA596500.049.4941372
GGTTAGA620150.048.530564
TTACATT161300.046.140552
TGTACTC122450.044.7850232
GAACCTC670250.044.1527339
TTGTACT125350.043.5167161
TAGAACC689000.043.231047
CTGGGTT770850.040.058661
CGAGTAA200450.039.6613161
CATTCAG190500.039.3123255
TAGTTGT138800.039.169329
TGTCCTA206300.038.9748575
GGTCACA77150.038.8308371
AGAACCT774700.038.234288
TCTAGTT146700.037.996077
ACATTCA199050.037.9456374
GTTAGAA808550.037.650365
GGGAAAA126550.037.301014145
Produced by FastQC (version 0.11.5)