FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_8721.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_8721.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20861423
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306470.14690752399776372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA259900.049.466858145
TTGTACT121500.047.2835161
CCGTATA47450.046.151157145
TGTACTC129550.045.0744862
GACATAC80000.042.9150169
TCTAGTT147550.041.2192577
AACAGAC102350.034.385665
TACTCTA181850.032.7151534
AACGTAT225900.031.7699156
TGCCAGT119150.030.3184952
GAACGTA241100.029.7962365
CGTGTAT136100.029.729525145
GGGAAAA202300.028.711014145
GTTACGA86600.028.5683334
ACGTATT254700.028.5518447
TAGCATA130250.028.418318
GTAGCAT132050.027.4256867
GTAGATA294950.027.043081145
GTACTCT221500.026.8581453
AGACATA148350.026.7122768
Produced by FastQC (version 0.11.5)