FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_8404.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_8404.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23693866
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC519910.21942810008294972No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG351670.1484223807123751No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334430.14114623590763956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT91950.056.3326843
CGATCTA122650.045.662354
ATCTATT148950.041.553616
CTATTAG182650.037.0303658
CTCGATC142050.036.5100752
TCGTGTT137350.029.036201145
TGTCCTA172000.028.306075
AACGTAT631350.028.2840526
GATCCTG175400.027.3850755
ATGCCGT122600.026.8144868
GTATTCA677000.026.5967869
CGTATTC677400.026.5899988
GGAACGT634300.026.5453764
CCGGGAA610700.026.4020371
GATCTAT226900.026.2539225
TGGGCTA170750.026.2528042
CCATTGT371450.025.846145
TATTAGT263950.025.6262669
CGGGAAC636500.025.5040762
GAACGTA732100.024.5494165
Produced by FastQC (version 0.11.5)