FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_8336.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_8336.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23340594
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245880.10534436270130915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG184250.037.97874145
GAGTGTA211350.031.15332145
GTAGATA315350.028.927341145
CCGGGAA241000.026.5469571
CCGTATA89550.025.669321145
CGGGAAC311200.024.0781961
AACGTAT337700.023.1863045
GAACGTA350300.022.9286634
GGGAACG333250.022.3777732
GGGAAAA299500.022.274693145
GGAACGT336200.021.667563
TGTGTAT142250.021.410053145
TATTCAC361550.021.361279
ACGTATT369300.021.085096
GTATTCA391700.019.734318
TCGTGTT189250.019.426422145
CGTATTC408900.018.7260177
TGTCCTA105500.018.3653815
TGTCTTA244350.017.568314145
CCTACTC155650.016.787248
Produced by FastQC (version 0.11.5)