FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_8336.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_8336.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24334133
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG362870.14911975700962923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGC40200.039.355947
GACATAC57000.033.9982459
AACGTAT340350.031.6620836
GAACGTA348750.030.7319325
ACGTATT365950.029.549117
TGTACTC66450.029.1482242
GAGTGTA149350.028.356083145
TTGTACT66650.027.9728581
TGCCAGT126700.027.7690452
GTAGCAT97700.027.6330137
GTTACGA103200.027.136594
CCGGGAA376300.027.0860651
CGGGAAC410400.025.365322
CGTATTC438400.025.3450668
CCATTGT299550.024.741167145
TACGACT120300.023.7064046
GCCAGTA159850.022.421593
AACAGAC85750.022.171285
TGTGTAT56000.021.755037145
TAGGCTC76200.021.7170099
Produced by FastQC (version 0.11.5)