FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_6331.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_6331.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22797153
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300440.1317883860322383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG123350.039.7110185
CTCATAT53100.028.9621261
CAATTAG113800.027.874859
TTTGATC192400.027.2996392
TCCTGGC197400.025.5170967
CTGGCTC206850.025.2668069
TCACGAG98400.025.2147332
GGCTCAG173850.025.125014
TCGAGCT67250.024.598733
CCTGGCT198400.024.475318
ACGAGCA99600.024.3316524
CTATTAG139550.021.7415358
TCAGATT118950.021.4781637
GGGTTCA279250.021.0294889
ATCCTGG257650.021.0120536
GAGCAAT142950.020.9664886
TGATCCT225300.020.8366324
CGCGAGC300900.020.8054751
GCTCAGA139200.020.5922745
CATATCA64050.019.9371783
Produced by FastQC (version 0.11.5)