FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_6215.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_6215.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22187364
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5102542.299750434526607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282030.12711289182437355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT79900.059.716953145
AGTGTAT77900.036.675606145
GTACTCT107500.033.946163
TAGTTGT133350.026.3981749
GCCTAAC109500.025.9672321
CCTAACT119000.025.1748312
TGTACTC145750.024.9340422
TCTAGTT159350.023.9559697
GAGTCTA51400.022.8661884
TTGTACT157800.022.7996621
GCCATAT36300.021.5809651
TCAGATT126700.021.3548132
TACTCTA172900.021.1903574
GATCCAA101150.020.302375
TCTCTTG75750.020.2127765
TCATTAG70700.018.9838123
TGCTTGG144550.018.526838
GGGAAAA197750.018.371296145
CTAACTG149050.017.9121193
CATTAGA98000.017.3269396
Produced by FastQC (version 0.11.5)