FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_5764.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_5764.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22807312
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357620.15680059096837012No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC298590.13091854051016621No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC244420.10716738561738447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA224450.032.855595145
AACGTAT483200.032.4061166
CCGGGAA434150.030.9749341
CCATTGT336350.030.354229145
ACGTATT522100.030.037497
GAACGTA531450.029.5856325
CGTATTC560800.027.8752828
CGTGTAT101700.027.59294145
GTATTCA575350.027.2734139
GGAACGT518800.027.100494
GGGAACG563900.025.3569723
TGTACTC116900.024.5714952
GGGTTAG224900.024.5475443
CATTGTA219300.024.203625145
TTGTACT116550.024.1468161
CGGGAAC576850.023.6399142
GGTTAGA258050.022.294244
TGGGCTA176150.018.8596552
TAGTTGT149350.018.1267349
GTTAGAG111600.017.9444125
Produced by FastQC (version 0.11.5)