FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_31013.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_31013.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15943437
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3552082.2279261366291347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216790.13597444515884496No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT166420.1043815082030305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT84850.051.2191853
TACTCTA89750.048.5047234
TCTAGTT95400.045.5720377
TTGTACT96850.044.1876641
TGTACTC100550.042.780632
TAGTTGT103150.041.5908979
GCCTAAC69500.039.4507831
CCTAACT77750.036.5729682
CTCTAGT129250.033.9132276
GTAGATA73900.033.167118145
ACTCTAG145750.030.8194125
TAACTGC89350.030.6135754
GTCTAAC25450.028.2237454
CGATTAG50900.028.0927588
GAGTCTA31050.027.8009642
ATCGATT53250.027.5292556
GTTACTA44550.026.7087673
AGTGTAT36400.026.097889145
AGTCTAA34100.025.7447533
GCTTGGA107100.025.1457429
Produced by FastQC (version 0.11.5)