FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30982.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30982.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21469544
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG697520.32488812990159455No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC651900.30363942522486737No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA400330.1864641372914115No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA360080.16771665015335213No Hit
CCTCGATCTATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC303980.14158661217956003No Hit
CTGATATCGACCGATAGCGCATAGTACCGTGAGGGAAAGGTGAAAAGAAC252780.11773887698779256No Hit
AATCAAACGAGATTTTCTTAGTAGCGGCGAGCGAACGGGAAAGAGGCCAA241110.11230327015795026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222240.10351407556676565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCT69400.079.762123
CTCGATC74650.073.753192
CGATCTA96800.058.6855554
GCAATTA102150.055.704168
ATCTATT111600.052.9954346
GATCCTG109900.051.172295
TATTAGT143600.050.1910139
CTATTAG145600.047.2061428
ACGAGCA120800.045.1042864
GGGCACA19400.044.121491
GAGCAAT132950.042.5200926
CGCTGAT62650.042.0296131
AGCAATT139050.040.5550357
CAATTAG144150.039.979479
CGAGAAC157150.039.7889751
TGTCCTA19750.039.681455
CGAGCAA144200.039.5537955
CCGGGAA433050.039.1965371
GGCTCAG146100.035.853524
TCAGATT71250.035.345927