FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30979.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30979.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22127725
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA556550.25151704479335313No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG522540.23614718639173252No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC378210.17092132155474635No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT351670.15892731855624562No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG331230.14969003817608906No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG230170.10401882705971807No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC224070.1012621044413739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221350.1000328773066368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA212400.047.4853744
GGGTTAG233000.043.2237323
TGGGTTA235600.040.6442382
AACGTAT382700.035.272215
TTCGTTC151700.034.0709849
TATTCAC413600.032.5927969
GTATTCA415100.032.2981648
GAACGTA428550.032.2716334
ACGTATT420850.032.1962286
GTTAGAA304200.031.4198975
GAACCTC299550.031.213099
CGGGAAC432950.030.612321
GGAACGT432950.030.434553
TTAGAAC332950.029.1216166
ACGTCCG86000.028.415157145
CATTGTA185400.027.925894145
CTGGGTT409550.027.8800281
GGGAAAA170400.026.42656145
CTCCGAC178500.026.3755721
TAGAACC362300.026.0044367
Produced by FastQC (version 0.11.5)