FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21729389
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284920.13112195653545528No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT238320.10967634662898253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA69850.028.3575884
CGAGCAA90400.023.922195
TCACGAG90750.021.9024622
TCGTGTT117850.020.427324145
AACGTAT189950.020.1725466
GTTACGA60150.019.5412544
AGATTAT265300.018.995491145
ACGTATT205050.018.618567
TACGACT65900.018.3906546
GATCCCG203450.018.2233523
GAACGTA216950.017.6280425
CAATTAG136400.017.5622359
TTACGAC68550.017.2557285
CGTGTAT70850.017.19382145
ATCCCGA218350.017.0465774
TACGGAC172300.016.9314385
CCGTATA30200.016.567047145
CGGGAAC225550.016.5635492
CGTATTC245900.016.0284488
CCGGGAA243250.015.6260941
Produced by FastQC (version 0.11.5)