FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30978.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30978.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24229767
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323790.1336331463690922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT109650.065.27075145
GACATAC55150.036.4559829
GAGTCTA85350.035.2718162
TGTACTC89250.033.486692
TTGTACT88800.032.9215131
GTTACGA92750.032.7766154
AGTGTAT108600.032.11629145
TCGTGTT118950.031.089651145
AGTCTAA98700.029.9177953
GTAGCAT118600.029.1285067
TGCCAGT116400.028.8543452
TTACGAC105900.028.6425465
TAGCATA126350.027.457628
CCGTATA36400.026.494783145
TACTCTA118150.026.037314
TGTTACG116500.025.9071583
GTCTAAC119700.024.6091084
GCCAGTA142800.024.5906223
TCTAGTT129550.024.3695667
TCTAACA121350.024.3380585
Produced by FastQC (version 0.11.5)