FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30976.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30976.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21985385
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG357300.16251705394288068No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT355550.1617210706112265No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT342030.15557153081467529No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT285000.12963157115511054No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT247830.11272488519077559No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC233470.10619327339503037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC42850.037.9424139
GTACGTT30100.037.584354145
TCGTGTT93350.037.2109145
GAGTCTA70050.036.244032
TAGCATA80700.032.9165658
GTAGCAT81600.032.552337
AGTCTAA77900.032.503253
TGTACTC54300.032.3289832
CGTGTAA31150.032.12699145
GTTACGA70100.031.6703684
TTGTACT55750.030.8371371
TTACGAC74150.029.8472045
TGCCAGT86200.029.7901382
CGGATGT39400.028.9050711
CCGTATA25550.028.382988145
TCTAACA91650.027.6317215
GGCCAAC219900.027.3793721
CCAGTAG96250.027.1363534
GTCTAAC94700.026.7377684
CGCTCAG69400.026.6581754
Produced by FastQC (version 0.11.5)