FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30908.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30908.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23883387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323530.13546236134765977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT147400.018.94019145
AGAGCGG114350.016.48763145
CGTGTAT95500.013.21195145
GTAGATA229300.012.83935145
CGGGAAT93900.012.4371941
GGGGAAT201450.012.2065951
CGGGGTT158200.012.1965571
TCTCGGT279400.011.497366145
TCGGAAA127500.010.862861145
CTCGGTG296850.010.748222145
AGGGTGT779400.010.671466145
CGGGGAT91150.010.6637571
CGTCGTT102050.010.587522145
GATCTCG368050.010.520963145
TCGGTGG280900.010.3517475145
GATAGGG943650.010.281731145
GTGTAGA618000.010.126127145
ATCTCGG351100.010.120135145
GGGTGTA660050.010.118214145
ATAGGGT840850.09.865715145
Produced by FastQC (version 0.11.5)