FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30904.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30904.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21562213
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1407690.6528504286642564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG321760.14922401517877593No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG270480.12544166964680295No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT246640.11438529060073752No Hit
GCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCC239020.1108513305197384No Hit
GGAATATTAACCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGG236070.10948319636764556No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC221700.10281875983694252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT61000.075.48628145
GATCGAT68950.068.516875
ATCGATT69100.068.475696
TCGATTA69400.068.081137
CGATTAG69650.067.317478
TCGATCG77100.060.6040423
AGTGTAT46550.059.506966145
CGATCGA83450.057.036874
GGGAAAA74900.051.408726145
GTAGATA58900.050.476902145
TTGAACG62850.049.6556247
GATTGAA63100.049.222675
GATCCAG127850.049.2117925
TCAGATT61550.049.1469352
ATCCAGC130750.047.789056
TCTAGTT28100.047.5244147
GCGACTA35150.044.5776182
CCTAACT85600.044.4911882
GTACTCT32000.043.9867483
GGAAGCA111450.042.4460833
Produced by FastQC (version 0.11.5)