FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30903.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30903.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22843571
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340370.1490003467496391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTT136050.038.909245145
GACATAC80150.036.3166859
AGATTAT303500.035.863323145
AGTGTAT93900.034.9061145
TTGTACT82150.032.848691
TGTACTC85450.032.091992
AACAGAC96600.030.0494045
TCGTGTT122200.027.94977145
CCGTATA40800.027.726366145
CGGATGT72750.026.1246761
GGGAAAA232950.025.74372145
GTAGCAT113150.025.5293987
TGCCAGT112600.025.3848572
GTTACGA86900.025.2982884
TGTTAAT215150.024.941288145
TAGCATA116850.024.9083968
GCGTTCC77000.023.3729725
TTACGAC99700.022.3458185
AGACATA156250.021.8327278
GTAGATA328150.021.14794145
Produced by FastQC (version 0.11.5)