FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30893.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30893.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20697435
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245900.11880699226739931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA86750.061.103428145
TGTGCCG173900.051.622463145
CTCTATT266350.049.848633145
GTAGATA400650.043.67268145
CTATGTT186850.040.7099145
TGTGTAT189100.037.081097145
CCGGGAA165450.032.4873281
GTGCCTA183750.030.228674145
TGTGCCA280850.028.504425145
CGGTGGG119450.027.439104145
CGGGAAC223550.025.9908431
AACGTAT229200.025.962515
GAACGTA252900.024.9024244
GGAACGT235650.024.8155333
GGGAACG238700.024.6153872
TCGTGTT168350.023.087095145
ATGTGTT211900.022.551325145
ACGTATT267050.022.419326
TATTCAC285000.020.6557589
GGGAAAA351000.020.287195145
Produced by FastQC (version 0.11.5)