FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30893.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30893.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19512136
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC532560.2729378270016158No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC303970.155785097028844No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG268690.137704042243248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262290.1344240323048179No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT249790.12801776289382158No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT213740.1095420819125082No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG204580.10484756768813008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA500500.038.520831
TGTGTAT37600.036.83229145
GCTGTAT39650.035.8556481
CCATTGT385550.035.61906145
GATCCTG79750.035.3020135
AACGTAT624350.034.0993466
GAACGTA676550.031.6817425
ACGTATT691650.031.0367347
CGTATTC716850.030.017628
GGGAACG690150.029.9050663
TGGATGC152350.029.4750233
CGGGAAC668900.029.3637142
CATTGTA253300.029.111797145
GGAACGT721450.028.5180914
GGGAAAA164900.028.229136145
GTATTCA770250.028.0985329
CAATTAG76600.027.761739
GTTAGAG147950.027.758755
GGCTCAG122500.027.0645354
GGATGCC188800.025.3607674