FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30890.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30890.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23535881
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG472180.20062134066704368No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC424970.18056260566579174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274790.11675364945973343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA72750.052.32838145
CGATCTA51750.047.5288284
TGTGTAT111450.043.52683145
GAGTGTG197300.042.412094145
TGTCCTA117700.040.691055
CCGGGAA300750.038.9042171
CTATTAG111500.038.3400968
GAGTGTA262350.035.516735145
CGGGAAC412700.033.219671
GTGTCCT145450.033.1723064
CCTACTC152700.032.891218
GGAACGT403500.032.5999153
GGGAACG426750.032.2464642
AACGTAT480500.031.7899745
TGGGCTA161100.029.9443152
CTACTCT170450.029.425479
GTATTCA530400.028.7908868
TGTGTAA82550.028.723785145
TTCGCCG220150.028.622795145
GAACGTA567900.028.0178284
Produced by FastQC (version 0.11.5)