FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30890.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30890.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20355190
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT368750.1811577293063833No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT326910.1606027750170841No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309070.15183842548264104No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT280060.13758653198520868No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT248160.1219148531652124No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG245210.12046559133076133No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA224170.11012916116233747No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT223680.10988843631525916No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC222650.10938242286119657No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG213650.10496094607812553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG56350.049.799294145
CGGATGT34300.040.1823541
CTCGGCT78100.037.2451061
CGCTCAG74500.035.8369034
GACATAC42250.033.843819
CCGTATA24600.032.423786145
TGCCAGT75050.032.0883452
GAACGTA181300.030.4580945
GAGTCTA56650.030.3464782
AACGTAT187750.029.6832926
ACGTATT190050.029.5570837
GTTAGAG92600.029.228985
TCGTGTT102950.028.66647145
TGTACGG63550.028.525322145
GCCAGTA90000.026.601433
GGGAAAA180950.025.606392145
GGCCAAC185400.025.5101931
CGTATTC225650.025.3775318
TAGCATA86450.024.9344128
TTACGAC66850.024.8570695
Produced by FastQC (version 0.11.5)