FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30889.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30889.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22829979
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397530.1741263099716386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT112350.041.9021341
TGTACTC119550.039.4395562
TCGTGTT163550.037.818974145
GAGTCTA78850.032.106432
TCTAGTT161900.030.9304857
GACATAC53300.030.6390979
TACTCTA166400.029.1248574
TGTGCCG226950.028.11666145
GTACTCT176400.027.4316983
TTACGAC82750.027.1836435
GTTACGA82750.027.0037064
AGTCTAA93000.026.6009333
TAGTTGT194200.024.59229
GTCTAAC105800.023.3149174
TCTAACA109900.023.109175
GAACGTA137700.023.0809765
TGTTACG96900.023.0596313
TAGCATA110100.022.741958
GTAGCAT112950.022.1675327
GTAGATG105450.022.073421145
Produced by FastQC (version 0.11.5)