FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_30887.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_30887.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20505104
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770401.3510782486155644No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG283470.138243629488541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269730.1315428587926206No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG206250.10058471295732029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT68650.046.393293
TACTCTA78350.041.4841234
TGTACTC77350.041.3566972
TTGTACT77250.041.0348321
ATCGATT63850.041.0288056
TCTAGTT78750.039.3521657
TAGTTGT79700.038.5229039
CCTAACT75950.037.9166832
GCCTAAC82050.034.9211241
CGATTAG74150.034.551818
GATCGAT78250.034.2186055
TCGATTA79750.032.2154277
TCATTAG67100.030.9225083
TAACTGC95900.029.2775844
TCAGATT60800.027.9177152
TTGAACG55000.027.8426847
GGAAGCA99500.027.0509193
GCTTGGA104200.026.8878779
GTAGATA72900.026.259668145
ATCCAAC76200.025.9033836
Produced by FastQC (version 0.11.5)