FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n02_2397.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n02_2397.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21522125
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4956302.30288598360989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290970.13519575785383645No Hit
CTGGAAGCAGGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACG264600.12294325025990695No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT250690.1164801338157826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG120100.054.3765265
CTCTAGT122400.052.7052086
GTACTCT124050.051.9931643
TCTAGTT130950.049.269597
TACTCTA134650.048.6549074
TAGTTGT130900.048.463769
TTGTACT135650.047.6456681
TGTACTC135150.047.393112
CGATTAG54450.041.720588
GTACGTT23300.035.78836145
GCCTAAC92300.033.636251
CCTAACT93050.033.365522
ATCGATT72750.033.3155256
TCGATTA68600.033.2191287
GGAAGCA141000.032.6735153
GTAGATA89850.029.778866145
GATCGAT87300.029.3408325
GCGACTA43650.027.7522181
TCATTAG82650.027.1241953
GATCCAG160550.026.9369165
Produced by FastQC (version 0.11.5)