FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41633971
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32859297.892422752564246No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1811340.43506299218971933No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1657980.3982276876736067No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1082130.2599151543819829No Hit
CGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555820.13350155813866518No Hit
GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532350.12786433463192834No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484400.11634729725876977No Hit
CGGGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447660.10752277268963846No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA447410.10746272557090458No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416580.10005771488864226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTC242000.033.3027232
GGGTTCT303900.032.8741463
CGGGTTT279200.032.0802151
CGGGGTT359750.029.8687171
GCTGTGT123950.028.334371
CTTTTTT1049400.027.4609491
CCCCTGT114500.021.16241
CCGGGGT335550.020.8900811
GGGTTTT559000.020.7103312
GTTCTTT568000.020.6805535
GGTTCTT598100.020.189874
CTGTGTT331600.019.7172372
CCGGGTT398200.019.0159991
GAACGTA306100.017.419565
CCGGGAA306700.016.5535071
CGGGAAC301550.016.3034062
CCATTGT229500.015.375412145
TGTACTC80800.015.2559082
GGAACGT334550.014.9120584
TTGTACT81400.014.8838741
Produced by FastQC (version 0.11.5)