FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_9424.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_9424.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21214209
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA1477190.6963210365279233No Hit
CGAGTAATGGGGGATAACTAATCGAAAGATTAGCTAATACCGCATATTCT544490.2566628809964114No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG544400.2566204566005737No Hit
CTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAGGATAGGT480050.226287013576608No Hit
GTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCACAGA385240.18159526947245594No Hit
CGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGG381230.17970502694679777No Hit
CGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGT379950.1791016577615503No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC344740.16250429134548453No Hit
GCGGTGTCCTACTCTCCCACAACCCATCGGTTGCAGTACCATCGGCGCTG265180.12500112542494515No Hit
CGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGTCACTTGTGTAG263670.1242893383392235No Hit
ATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTA239600.1129431693635148No Hit
TGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTA223460.1053350610432847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT156550.055.960221
TTACATT171850.050.6322942
GGGTTAG523500.045.804193
TGGGTTA520950.045.0410352
GGTTAGA543650.044.2532044
ACATTCA200550.043.6749084
CATTCAG202050.043.567015
CGAGTAA197300.042.417421
GGTCACA77700.041.1607321
GAACCTC580800.040.798679
ATTCAGT219050.040.68246
TAGAACC594950.039.85277
TGTCCTA186400.038.7446175
GTTAGAA684100.035.4653555
TTAGAAC688750.035.3101356
TGTACTC132850.035.0950052
AGAACCT675050.035.091658
CTGGGTT697750.034.8705021
TACATTC253900.034.8119773
TTGTACT135350.034.505881
Produced by FastQC (version 0.11.5)