FastQCFastQC Report
Sat 13 Jul 2019
HMJ53BGXB_n01_8721.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMJ53BGXB_n01_8721.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20861423
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACAGACATACTCCCCGAAGGAAGTGCAAGATGCGTTCAAAGTTTT216370.10371775693345558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC78850.044.2194489
AACAGAC99950.035.9087755
AGTCACA96150.032.509193145
TGCCAGT124500.030.2250392
GTTACGA88550.029.8858664
TAGCATA133700.028.5725618
TTGTACT120600.028.4419061
GTAGCAT134400.028.261947
CGGATGT67850.027.8955881
AACGTAT224050.027.7664626
TGTACTC125300.027.5438582
GAACGTA229200.027.110935
TTACGAC97950.027.092865
AGACATA150850.027.0060428
TCTAGTT143650.025.987927
GCCAGTA151900.025.3453523
ACGTATT251250.025.215957
CTCCAAC166850.024.6869221
CTGCCAG155300.024.5150471
ACACGTA57200.024.343521145
Produced by FastQC (version 0.11.5)